RAD51: Beyond the break
نویسندگان
چکیده
منابع مشابه
RAD51-dependent break-induced replication in yeast.
A chromosome fragmentation assay was used to measure the efficiency and genetic control of break-induced replication (BIR) in Saccharomyces cerevisiae. Formation of a chromosome fragment by de novo telomere generation at one end of the linear vector and recombination-dependent replication of 100 kb of chromosomal sequences at the other end of the vector occurred at high frequency in wild-type s...
متن کاملTODRA, a lncRNA at the RAD51 Locus, Is Oppositely Regulated to RAD51, and Enhances RAD51-Dependent DSB (Double Strand Break) Repair
Expression of RAD51, a crucial player in homologous recombination (HR) and DNA double-strand break (DSB) repair, is dysregulated in human tumors, and can contribute to genomic instability and tumor progression. To further understand RAD51 regulation we functionally characterized a long non-coding (lnc) RNA, dubbed TODRA (Transcribed in the Opposite Direction of RAD51), transcribed 69bp upstream...
متن کاملDouble-strand break repair in the absence of RAD51 in yeast: a possible role for break-induced DNA replication.
In wild-type diploid cells of Saccharomyces cerevisiae, an HO endonuclease-induced double-strand break (DSB) at the MAT locus can be efficiently repaired by gene conversion using the homologous chromosome sequences. Repair of the broken chromosome was nearly eliminated in rad52delta diploids; 99% lost the broken chromosome. However, in rad51delta diploids, the broken chromosomes were repaired a...
متن کاملGas exploration beyond the shelf break: An oceanographic challenge
Norway's second largest gas field, Ormen Lange, is located 140 km west off Kristiansund at an unprecedented depth when it comes to exploration. It will be the first Norwegian project beyond the shelf break. Exploration and development of the field is thus a challenge. An important issue during the planning stage is to understand the current conditions and hydrography of the site. This is especi...
متن کاملCdk1 uncouples CtIP-dependent resection and Rad51 filament formation during M-phase double-strand break repair
DNA double-strand break (DSB) resection, which results in RPA-bound single-stranded DNA (ssDNA), is activated in S phase by Cdk2. RPA-ssDNA activates the ATR-dependent checkpoint and homology-directed repair (HDR) via Rad51-dependent mechanisms. On the other hand, the fate of DSBs sustained during vertebrate M phase is largely unknown. We use cell-free Xenopus laevis egg extracts to examine the...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Seminars in Cell & Developmental Biology
سال: 2021
ISSN: 1084-9521
DOI: 10.1016/j.semcdb.2020.08.010